Class II, Type B2 Biosafety Cabinet
Type B2 Biosafety Cabinet – 100% Exhaust for High-Risk Labs
Dyna Make Type B2 Biosafety Cabinet (BSC) is a Class II, 100% exhaust biosafety cabinet designed for biosafety levels 1, 2, and 3, ensuring absolute protection when working with hazardous biological agents, toxic vapors, and volatile chemicals. Unlike Type A2 BSCs, which recirculate a portion of the air, Type B2 BSCs exhaust all air through a hard duct system, preventing cross-contamination and ensuring maximum operator and environmental safety.
At Dyna Filters, we take pride in being among the top Class B2 Biosafety Cabinet manufacturers in India, delivering reliable and high-performance Biological Safety Cabinets (BSC) for critical lab and industrial applications.
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Key Features of Type B2 Biosafety Cabinet
- 100% Air Exhausted via Hard Duct – Ensures no recirculation of contaminated air.
- Negative Pressure Plenum – Prevents air leaks and protects the external environment.
- Ducting Options Up to 5 Meters – Allows flexibility in lab exhaust system integration.
- Inflow Velocity ≥ 100 FPM – Ensures safe and effective containment.
- ISO 5 Air Cleanliness Standard – Prevents particle contamination in critical applications.
- Designed for Hazardous Applications – Recommended for handling volatile chemicals, gases, and cytotoxic substances.
Product Specifications of Type B2 Biosafety Cabinet
| Specification | Details |
| Biosafety Level | BSL-1, BSL-2, BSL-3 |
| Airflow System | 100% exhaust – No air recirculation |
| Exhaust System | Hard duct connection (up to 5 meters) |
| Inflow Velocity | ≥ 100 FPM |
| Work Environment | ISO 5 Cleanroom Standard |
| Air Containment | Negative plenum prevents environmental fouling |
| Safety | Higher operator protection when handling toxic vapors and gases |
| Material | Corrosion-resistant stainless steel |
Applications – Where Type B2 Biosafety Cabinets Are Used
Chemical & Pharmaceutical Research
Safe handling of toxic chemical vapors and volatile substances
Toxicology & Biochemistry Labs
Prevents contamination when working with hazardous reagents
Radioisotope Handling
Secure containment for radioactive biological materials
Hazardous Drug Preparation
Used in oncology, chemotherapy, and pharmaceutical labs
BSL-3 Research Facilities
Critical containment for high-risk pathogens and hazardous materials
Why Choose Dyna Type B2 Biosafety Cabinets?
100% Exhaust for Absolute Containment
- No air recirculation – Eliminates contamination risks when working with hazardous fumes, gases, or toxic chemicals.
- Hard-ducted exhaust system for direct ventilation into the building’s air management system.
- Negative pressure containment prevents hazardous air leaks.
Enhanced Operator & Environmental Safety
- Unlike Type A2 BSCs, which recirculate air, Type B2 BSCs ensure 100% exhaust, making them ideal for chemical and pharmaceutical applications.
- Fully compliant with biosafety and chemical safety guidelines, protecting both users and the external environment.
Global Biosafety Standard Compliance
Dyna Type B2 BSCs are designed to meet the highest laboratory safety standards:
- EN12469-Certified – Compliant with European biosafety regulations
- NSF/ANSI 49 Approved – Meeting strict U.S. biosafety standards
- ISO 14644 Cleanroom Compliant – Ensures a contamination-free workspace
Frequently Asked Questions (FAQs)
What is a Class II, Type B2 Biosafety Cabinet?
A Class II, Type B2 Biosafety Cabinet (also known as a total exhaust cabinet) is a specialized biosafety cabinet designed to provide maximum protection for personnel, product, and the environment—especially in applications involving toxic chemicals and hazardous vapors. It features 100% external exhaust with no recirculation of air inside the cabinet.
How does a Type B2 Biosafety Cabinet work?
Air enters through the front grille and flows into the work area, where it is immediately drawn into exhaust HEPA filters. Unlike Type A2 cabinets, none of the air is recirculated. Instead, all contaminated air is safely vented outside through a dedicated duct system, ensuring zero chemical buildup within the cabinet.
What makes a Type B2 cabinet different from other Class II BSCs?
The key distinction lies in the airflow and chemical safety:
- 100% of air is exhausted externally.
- No air recirculation, unlike Type A2 or B1.
- Ideal for applications with chemical hazards, whereas A2 is limited to trace amounts.
What makes Type B2 different from Type A2 Biosafety Cabinets?
Type A2 BSCs recirculate 70% of the air and exhaust 30%, making them suitable for non-volatile biological work. Type B2 BSCs, on the other hand, exhaust 100% of the air through a hard duct, making them ideal for handling toxic chemical vapors, gases, and hazardous biological substances.
What types of research require a Type B2 Biosafety Cabinet?
Pharmaceutical testing, toxicology, oncology drug preparation, virology research, and BSL-3 lab studies typically require 100% exhaust biosafety cabinets to ensure total operator and environmental protection.
When should I choose a Class II, Type B2 cabinet for my lab?
A Type B2 BSC is the best choice if your work involves:
- Volatile toxic chemicals
- Radionuclides
- Hazardous chemical vapours
- Procedures that generate high levels of airborne contaminants
What type of ducting is required for a B2 Biosafety Cabinet?
A hard ducted exhaust system is mandatory for Type B2 cabinets. This includes:
- Connection to a dedicated exhaust blower
- Use of chemical-resistant materials
- Proper airflow balancing and alarms to detect failure
- Dyna Filters provides end-to-end support for safe and compliant installation of exhaust systems.
Do Type B2 Biosafety Cabinets require external ducting?
Yes. Type B2 BSCs must be connected to a hard-ducted exhaust system (up to 5 meters) for proper airflow and containment.
Can a Type B2 BSC be used for microbiological work only?
Yes, but it’s not necessary unless there’s chemical involvement. For standard microbiology or tissue culture, a Type A2 BSC is usually sufficient. Type B2 is over-specified for basic applications, making it more suitable for high-risk or chemical-intensive environments.
What are the safety features of Dyna’s Type B2 Biosafety Cabinets?
Dyna’s B2 cabinets include:
- HEPA-filtered supply and exhaust air
- Zero air recirculation
- Negative pressure plenums
- Front airflow alarms
- Compatibility with Building Management Systems (BMS) for monitoring
How often should I service or test a Type B2 BSC?
For continued safety, your B2 BSC should undergo:
- Annual certification as per NSF/ANSI 49 standards
- Routine airflow and filter integrity checks
- Periodic exhaust system balancing
Dyna offers AMC and compliance support for all critical lab equipment.
What certifications do Dyna’s Class II, Type B2 cabinets meet?
Dyna Filters’ B2 cabinets are built to meet global cleanroom and biosafety regulations including:
- NSF/ANSI 49
- EN 12469
- ISO 14644
- Compliant with BSL-1, BSL-2, and BSL-3 lab environments
Get a Quote for Type B2 Biosafety Cabinets
Ensure laboratory safety with Dyna Type B2 Biosafety Cabinets.
Contact us today for a customized quote or expert consultation.
- Request a Quote
- Call: 9370144760
- Email: sales@dynafilters.com