Class III Biological Safety Cabinet
Class III Biological Safety Cabinet: The Ultimate Protection for High-Risk Pathogens
Dyna Make Class III Biological Safety Cabinet (BSCs) are designed for high-risk biosafety level 4 (BSL-4) and (BSL-3) laboratories, providing the highest level of biocontainment for handling dangerous pathogens, infectious agents, and toxic materials.
Engineered for gas-tight operation, these cabinets feature negative pressure containment, ensuring a secure, airtight workspace for research and pharmaceutical applications.
At Dyna Filters, we take pride in being among the top Class III Biosafety Cabinet manufacturers in India, delivering reliable and high-performance Biological Safety Cabinets (BSC) for critical lab and industrial applications.
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Key Features of Class III Biological Safety Cabinet
- 100% Air Exhausted – Ensures absolute contamination control via a hard duct exhaust system.
- Compliance – one of the first in India to be EN12469 Certified.
- Gas-Tight Construction – Completely sealed chamber for maximum operator safety.
- Negative Pressure Containment – Prevents hazardous material escape.
- Dual HEPA Exhaust Filtration – Eliminates airborne contaminants before discharge.
- ISO 5 Work Environment – Maintains an ultra-clean, contamination-free workspace.
- Passbox Access Only – No direct entry, ensuring material containment.
- Completely Sealed View Window – Provides clear visibility while maintaining security.
- Glove Ports for Secure Handling – Prevents direct exposure to hazardous substances.
Product Specifications of Class III Biological Safety Cabinet
| Specification | Details |
| Biosafety Level | BSL-4 (also applicable for high-risk BSL-3 applications) |
| Air Exhaust System | 100% air exhausted through a hard duct connection |
| Cabinet Enclosure | Gas-tight, fully sealed stainless steel structure |
| Air Containment | Negative pressure operation in both cabinet & associated ducting |
| Interior Access | Pass boxes only – no direct access for personnel |
| Work Environment | ISO 5 cleanroom standard for contamination-free processing |
| Operator Safety | OPTIONAL Gloves attached to the front panel prevent direct contact with hazardous material |
| View Window | Fully sealed for maximum containment |
| Filtration System | Two-stage HEPA exhaust filtration to remove hazardous particles |
Why Choose Dyna Class III Biological Safety Cabinet?
Maximum Containment & Operator Protection
- Completely sealed, gas-tight chamber for zero exposure to pathogens.
- Negative pressure system prevents contaminated air leaks.
- Pass box-only material transfer to ensure containment integrity.
Compliance with Global Biosafety Standards
Dyna Class III BSCs meet and exceed global safety regulations, including:
- EN12469 Certified – Europe’s gold standard for biosafety cabinet performance.
- NSF/ANSI 49 Compliant – Ensuring the highest safety levels in the USA.
- ISO 14644 Certified – Compliance with international cleanroom standards.
Class III Biological Safety Cabinet Applications:
BSL-4 & BSL-3 Laboratories
Handling high-risk pathogens like anthrax, Covid, Ebola, and SARS.
Pharmaceutical Research
Biotechnology & Virology Research
Military & Biodefense Labs
Animal Testing & Infectious Disease Control
Frequently Asked Questions (FAQs)
What is a Class III Biological Safety Cabinet?
A Class III Biosafety Cabinet is a gas-tight, fully enclosed safety cabinet designed to offer maximum protection to personnel, product, and the environment when handling hazardous or highly infectious materials. All operations are conducted through attached gloves, ensuring no direct contact with the work environment.
How is a Class III BSC different from a Class II BSC?
Class III BSCs provide total containment with a gas-tight, glove box system, whereas Class II BSCs offer operator, product, and environmental protection but do not provide full containment of hazardous pathogens.
What is the purpose of negative pressure in Class III BSCs?
Negative pressure ensures that no contaminated air escapes into the lab environment, maintaining maximum safety for operators.
What industries require a Class III Biosafety Cabinet?
Virology research labs, pharmaceutical companies, military biodefense, high-security diagnostic centers, and vaccine production facilities require Class III cabinets for high-risk pathogen handling.
When should a Class III BSC be used?
Class III cabinets are ideal for:
- Handling BSL-3 and BSL-4 pathogens
- High-risk research involving viruses, bacteria, or biological toxins
- Work involving radioactive isotopes or toxic chemicals
- Pharmaceutical and API manufacturing of cytotoxic compounds
- Biodefense, vaccine production, and synthetic biology labs
How does Dyna’s Class III BSC ensure operator safety?
Dyna’s Class III Biological Safety Cabinet is designed to maintain a negative pressure environment, ensuring inward airflow at all times. Key safety features include:
- Gas-tight construction
- Dual HEPA filters for supply and exhaust
- Arm-length glove ports for sealed interaction
- Pass-through chambers for contamination-free transfer of materials
- Optional hydrogen peroxide (H₂O₂) decontamination compatibility
What is the airflow mechanism in a Class III Biological Safety Cabinet?
- Supply air is passed through a HEPA filter before entering the work zone.
- Exhaust air is filtered through two HEPA filters in series or HEPA + carbon filters when chemicals are involved.
- All contaminated air is safely ducted out through a building’s dedicated exhaust system, eliminating any risk of leakage into the room.
What makes Dyna’s Class III Biological Safety Cabinet unique?
Dyna Filters’ cabinet is engineered with:
- All-welded SS 304/316L construction for durability and cleanroom compatibility
- Rounded internal corners for easy cleaning and decontamination
- Customizable glove ports and front window layout
- Ergonomic design for user comfort during extended operation
- Smart control system for monitoring airflow, pressure, and alarms
Can the cabinet be integrated with isolators or sterilization ports?
Yes. Dyna’s Class III Biological Safety Cabinet can be integrated with:
- Filling and dispensing lines
- Sterile isolators
- Rapid Transfer Ports (RTP)
- H₂O₂ fogging ports for decontamination
- This allows seamless incorporation into pharma-grade or cGMP facilities.
How are materials transferred in and out of the cabinet?
The cabinet includes an airlock or pass-through chamber equipped with interlocked doors. Materials enter and exit the cabinet without compromising containment, ensuring zero cross-contamination between external and internal environments.
Is the cabinet certified or compliant with international standards?
Yes. Dyna’s Class III Biological Safety Cabinet is designed to comply with:
- EN 12469
- NSF/ANSI 49
- ISO 14644 (Cleanroom Classification)
- WHO Biosafety Guidelines
- Meets requirements for BSL-3 and BSL-4 operations
What sizes and configurations are available?
Dyna Filters offers custom-built Class III cabinets with:
- Variable widths and chamber depths
- Multiple glove port layouts (2, 4, 6 ports)
- Options for built-in viewing windows, lighting, and data ports
Customisation is done based on lab layout, operator workflow, and biosafety compliance needs.
Why should you choose Dyna Filters for Class III Biological Safety Cabinets?
Dyna Filters is a leading Indian cleanroom solutions provider offering:
- Precision-engineered Class III Biological Safety Cabinets with robust containment technology
- Custom design flexibility for specialised applications
- End-to-end service: design, installation, validation, and AMC
- Decades of experience serving pharma, healthcare, and research institutions
Get a Quote for Class III Biosafety Cabinets
Ensure the highest level of safety in your laboratory with Dyna Class III Biosafety Cabinets.
Contact us today for a customized quote or expert consultation.
- Request a Quote
- Call: 9370144760
- Email: sales@dynafilters.com